Identification of major PBMC subtypes by scRNA-seq
We analyzed the PBMCs from four TA patients and four healthy controls
(HC). After quality control, 8966 cells from the TA patients and 20976
cells from the HC group were further analyzed by scRNA-seq. After
analysis, five major cell clusters were identified, as shown inFigure 1a and Table 1 , including M cells (Cluster
0,4,6,8), T/NK cells (Cluster 1,2), B cells (Cluster 3,9),
megakaryocytes (Cluster 7) and unknown subset (Cluster 5).
The high expression of S100A8, LYZ, and S100A9 (Figure 1b, d)suggested that clusters 0, 4, 6, 7, and 8 are antigen-presenting cells
(APCs). High expression of PF4 and PPBP (Figure 1d) , suggested
cluster 7 to be megakaryocytes. Low CD14 (Figure 1c) and high
HLA gene expression (Figure 1d) suggested cluster 8 to be
dendritic cells (DCs). Both cluster 1 and cluster 2 were identified as
T/NK cells, because of the high expression levels of CD2, CD3D, NKG7,
and GNLY (Figure 1b, c, d) . MS4A1, CD79A, and CD79B in cluster
3 and ITM2C, TCF4, and CCDC50 in cluster 9 (Figure 1b, c, d)indicate that both of them are B cells. Cluster 5, with high expression
level of NEAT1 and XIST, is still unknown.
We broadly classified the clusters under three major cell types mainly,
M (Cluster 0, 4, 6, 8), T cells (Cluster 1, 2), and B cells (Cluster 3,
9).