Single cell RNA sequencing
Single-cell suspensions were then loaded onto microfluidic devices and
scRNA-seq libraries were constructed according to Singleron GEXSCOPETM
protocol by GEXSCOPETM Single-Cell RNA Library Kit (Singleron
Biotechnologies). Individual libraries were diluted to 4 nM and pooled
for sequencing. Pools were sequenced on an Illumina HiSeq X with 150 bp
paired-end reads.