CD14+ monocyte, GZMB+CD8+ T cell, CD56dimCD16+ NK cell, and naïve B cell were increased
significantly in TA patients’ PBMC
According to the cluster analysis in this study, we compared the
proportions of different types of cells in the TA patients and healthy
controls. We used χ2 test to analyze the differences
in the composition of M, T, and B cell populations between TA patients
and healthy controls by SPSS 20.0. The data showed that M (Cluster 0, 4,
6, 8) and B (Cluster 3, 9) cells increased significantly while T
(Cluster 1, 2) cell population decreased (P < 0.05)(Table 2, Figure S1) in TA patients as compared to healthy
controls.
In order to further analyze the differences in gene expression of TA
patients and healthy controls, we compared the composition of M, T, and
B cell subclusters (Figure S2a, b, c) . Major findings of our
study showed a significant increase in CD14+ monocytes
and a reduction in CD16+ monocytes to
CD1C+ DCs ratio in M cell types (P < 0.05).
Among the T cell populations, the proportion of NKT cells and
CD56dim CD16+ NK cells increased
while proportion of CD4+ T cells, APCs, low ribosomal
expressing NK cells, CIML, and CD56neg NK cells
decreased in TA patients as compared to healthy controls (P <
0.05). In the B cell population, the ratio of naïve B cells increased
while the ratio of plasma cells to pDCs decreased in TA patients as
compared to healthy controls (P < 0.05).